This course seeks to help you take advantage of structural information in your research. It gives short introductions on methods (X-ray, cryo-EM, NMR and AI methods), protein folding and interaction, teaches how to interpret the quality of experimental structures and predictions, and how to perform, interpret, and extend results from structure prediction methods
Day 1:
morning : lectures on experimental techniques, protein folding, and macromolecular interactions
afternoon : tutorials on ChimeraX, looking at macromolecular structures, understanding experimental data
Day 2:
morning : lectures on protein folding and interaction, and AI structure prediction algorithms
afternoon : tutorials on AlphaFold/EBI databank, AlphaFold3, AlphaFill, AlphaBridge
Day 3:
morning : lectures and examples on AI-driven protein structure prediction in research projects
afternoon : More on ChimeraX, discussion on real-life projects
Please note:
– There are limited spots available for the course. We encourage you to register promptly. You will be notified in January whether a course spot is available.
– Due to course assignments, please bring your own laptop.
Image credit: Razvan Borza
Anastassis (Tassos) Perrakis (PhD) is a group Leader at the Netherlands Cancer Institute and Oncode Institute, and affiliated professor at Utrecht University. His lab develops methods for structural biology with a focus on safeguarding the quality of experimental structure models and to optimise the use of predicted structure models in research. At the same time we use X-ray crystallography, cryo-EM, NMR and other biophysical approaches to understand molecular mechanisms that are key in maintaining “healthy cells”, and contribute to the development of inhibitors of key proteins (e.g. mitotic kinases).
Titia Sixma (PhD) is a group Leader at the Netherlands Cancer Institute and Oncode Institute, and affiliated professor at ErasmusMC, Rotterdam. Her lab makes use of a combination of structural biology, biophysics and biochemistry to quantitatively analyze molecular mechanisms in cells, with an interest in DNA regulation by ubiquitin and DNA mismatch repair. Structural approaches such as cryo-EM analysis, X-ray crystallography and AlphaFold are combined with quantitative biochemical analyses to provide insights ubiquitin conjugation in the field of DNA regulation and repair.
