Biomedical proteomics

Function and structure of cells depend on the composition of proteins. During pathological conditions the expression of proteins may be altered leading to impaired function/structure of cells. Apart from changes in expression level, changes in post-translational modifications of proteins  may occur, resulting in altered activation state of signaling pathways. 

Proteomics based on mass spectrometry enables unbiased, large-scale analysis of proteins and their quantitative changes in health and disease. Biomedical proteomics is an activeresearch field in the health and life sciences that offers global insight into disease biology, thereby catalyzing discovery of novel biomarkers and targets for therapeutic interventions. 

Aims of the course:

• To provide PhD candidates with a global insight into the field of mass spectrometry-based proteomics, its theory and applications in biomedical and translational research. 
• To provide PhD candidates with hands-on experience with sample preparation for analysis by mass spectrometry.
  • To this end, you may bring your own samples (max two). Alternatively we can provide samples.  
• To provide PhD candidates with hands-on experience with functional data analysis.
  • Data will be generated using MS and database searching during the course.
  • You will analyze the obtained dataset for your own samples in a bioinformatics workshop using on-line tools (gene ontology mining and protein network analysis).